A FORMALIN-SUCROSE AMMONIA FIXATIVE FOR CHOLINESTERASES

A formalin fixative for immunochemical and ultrastructural studies on gastrointestinal endocrine cells.

Using indirect immunofluorescence, indirect immunoperoxidase, and unlabelled antibody enzyme techniques, gastrin, pancreatic glucagon, insulin, and somatostatin were localised in sections of both wax- and resin-embedded tissues that had been fixed in a buffered formalin solution. Ultrastructural preservation of the resin-embedded samples was also adequate for combined electron microscopy and li...

Will PAXgene substitute formalin? A morphological and molecular comparative study using a new fixative system.

UNLABELLED Formalin fixation and paraffin embedding present the standard procedures for conserving clinical tissues for histological analysis. However, molecular analysis is impaired by the cross linking properties of formalin. The PAXgene tissue system (PreAnalytix, Switzerland) is a new formalin-free tissue collection device. AIMS In this study we aimed to evaluate this new tissue preservat...

The New Formalin-free Fixative for Optimal Morphology and Molecular Analysis

Acetic acid-zinc-formalin: a safe alternative to B-5 fixative.

B5 fixation achieves superior morphologic detail. However, environmental concerns have led to labor-intensive and costly requirements for disposal of mercury-containing fixatives. We performed a blinded prospective study to find a safe, mercury-free alternative to B5. Morphology was evaluated with 6 fixatives, including B5, in a blinded fashion. Acetic acid-zinc-formalin (AZF) was selected for ...

Permanganate—A New Fixative for Electron Microscopy

During the early phases of tissue sectioning for electron microscopy, virtually all of the standard cytological fixatives were tried. Of these, osmium tetroxide appeared to give the best preservation, and this was further improved by buffering the fixative solution at a slightly alkaline pH (Palade (1)). A variant developed by Dalton (2) contains Os04 in a chromate-dichromate system. Formalin h...